A band of 95-110kD has been detected in brush border membrane (2)
ELISA:1:10,000-50,000 .
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide: S1010-87A
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a liquid, neat serum, 40% glycerol.
Specificity:The rat peptide sequence is ~67% conserved in mouse SGLT2. No significant sequence homology exists with other SGLTs. For human SGLT2, we recommend the use antibody that is made to the human SGLT-2 peptide. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Rt
Immunogen:A 16 AA peptide sequence from the N-terminal, cytoplasmic domain 1 of Rat SGLT-2 (1) was synthesized, coupled to KLH