Serotonin plays important physiological functions at the intestinal level.
Human intestinal epithelial Caco-2 cellls functionally express SERT both at their apical and basolateral cell membranes.
It is a 630aa residue in human (chromosome 17q11), rat and mouse.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:Recognizes rat SERT
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Hu Rt
Immunogen:Synthetic peptide corresponding to 15aa of rat SERT. This region is presumably from the extracellular domain. Epitope: N-terminus. Species Sequence Homology: human, monkey and mouse; 100%, guinea pig; 93%