Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting 1-5ug/ml for affinity pure using Chemiluminescence technique.
Resistin is ~9kD
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Affinity purified over the control-peptide Sepharose
Form：Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Specificity：Mouse sequence is only 42% conserved in human RELM-beta. We recommend the use of antibody that is raised to human RELM-beta sequence. No significant sequence was found with resistin or RELM-alpha. Antibody crossreactivity in various species is not established. We recommend the use of control peptide (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity. Control peptides, because of their small size (< 2kD), are not suitable for Western.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：A 14-aa peptide sequence near the N-terminus of Mouse RELM-beta (1) was coupled with KLH (carrier protein)
