RELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1 (prepeptides: mouse/rat 111aa; ~77% identity) is 63% related to resistin at the C-terminus (38aa).
The N-terminus of RELM-alpha is less conserved at the N-terminus.
RELM-alpha mRNA was most abundant in white adipose tissue.
It is also expressed in mammary tissue with significant fat pads.
Unlike resistin, RELM-alpha is not expressed in 3T3-L1 adipocyte or in preadipocytes.
It is also found in heart, lung and tongue, where resistin is absent.
In mice, RELM-alpha/FIZZ1 is found at low levels in a subset of bronchial epithelial cells and in non-neuronal cells adjacent neurovascular bundles in the peribronchial stroma, and in the wall of the large and small bowel.
FIZZ1 expression is induced hypertrophic, hyperplastic bronchial epithelium.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-5ug/ml (ECL)ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:R1587-03: Resistin-like Molecule, alpha, Mouse, Control Peptide (RELMa)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes mouse Resistin-like Molecule, alpha (RELMa). Species sequence homology: rat, 50%. No homology to human Resistin-like Molecule, alpha.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A synthetic peptide corresponding to 14aa near the N-terminus of mouse RELM-alpha. conjugated to KLH.
