IntroductionThe calcitonin family peptides probably act through G-protein coupled membrane receptors.
The gene for calcitonin receptors has been cloned.
It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP).
Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified.
CRLR has 55% homology with calcitonin receptor.
It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.
RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus.
RAMP1-3 displays 31% identity.
RAMPS may be involved in the transport of CRLR to the plasma membrane.
RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor.
RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract.
CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors.
RAMP2 (human 175 aa; rat 182 aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor.
It is expressed in the lung, breast, immune system and fetal tissues.
RAMP3 is most abundant in the kidney and lung.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using ECL
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide: R1060A
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.d sections of tissues may be tested.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The peptides are 52% and 91% conserved in mouse and rat RAMP1, respectively. However, no significant sequence homology is detected with other RAMPs. The control peptide, because of its small size (~2kD), is not suitable for Western. It should be used in ELISA, Dot Blot or to neutralize antibody and confirm specificity of antibody
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 19 AA Peptide mapping at the middle and an 11 aa peptide mapping near the C-terminus of human RAMP1 (1) were selected for antibody production. The peptides were coupled to KLH
