Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called “renal secretion”.
Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues.
OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
Prostaglandins and thromboxanes play important physiological, pathological, and therapeutical roles in health and disease.
PGT prostaglandin transporter also known as martin F/G), a member of oatp family, mediates transport of PGs (PGD2, PGE1, PGE2, PGF2a) from the circulation.
PGT (rat/human 643 aa; 12 TM) isexpressed in brain, stomach, ileum, jejunum, and kidney but not in heart or skeletal muscle.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
A band of 95-110kD has been detected in brush border membrane (2)
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Recognizes rat PGT. No significant sequence homology is detected with other oatp1-3, OATP or LST-1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：20aa peptide from the C-terminus region of Rat PGT (KLH).
