84-1048-33　Proline Transporter 100ul　P9010-97

特徴

• The regulated exocytotic release of neurotransmitters in response to neural activity requires storage within intracellular vesicles.
• In the nervous system, these vesicles are the synaptic vesicles that are derived from the endosomal compartment, whereas in endocrine cells larger secretory granules, such as the chromaffin granules of adrenal medulla, are derived from the trans golgi networks.
• For classical transmitters that are synthesized in the cytoplasm or appear there after removal form the synapses by plasma membrane reuptake, storage depends upon the active transport into the vesicles.
• Several distinct transport activities have been identified for monoamines, acetylcholine, glutamate, GABA and glycine.
• Vesicular monoamine transporters (VMATs) catalyze transport and storage of monoamines, serotonin, dopamine, norepinephrine, epinephrine, and histamine.
• The driving force utilized by the VMAT is the H+ electrochemical gradient generated by a vacuolar ATP-dependent H+ pump (V-ATPase) located on vesicular plasma membrane.
• VMAT is inhibited by a wide variety of compounds including reserpine and tetrabenazine.
• In contrast to the plasma membrane transporters for dopamine, norepinephrine, and 5-HT which show relative substrate specificity, the monoamine transporter recognizes various monoamine with similar affinity.
• It was therefore speculated that either similar or identical transporters mediate the monoamine transport in all the tissues.
• Rat Proline transporter is a high affinity sodium and chloride dependent L-Proline transporter.
• It is a 661aa, ~68-kD protein with 12 transmembrane domain.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000 using 50-100ng control peptide (Cat #P9010-99) per well
• Western Blot: 1:1000-1:5000 (ECL).
• Recognize a ~68kD band in rat and human brain.
• Deglycosylation reduces the MW to ~53kD
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.