Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides.
Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need.
In order to accomplish these complex tasks energy balance, adipocytes express many genes, including Pref-1, involved in lipid metabolism and glucose homeostasis.
Preadipocyte factor-1 (Pref-1), an epidermal growth factor-like domain-containing transmembrane protein is implicated in inhibiting preadipocytes differentiation.
Pref-1 (mouse 385aa, rat 383aa, human 383aa, chromosome 14q32), also known as fetal antigen 1 (FA1), Delta Drosophila homolog-like 1 (DLK1) or Zona glomerulosa-specific factor (ZOG), is synthesized as a membrane protein in preadipocytes but it is not detectable in mature adipocytes.
Dexamethasone, a promoter of adipocyte differentiation, down regulates Pref-1.
Pref-1 is proteolytically cleaved to generate a biologically active 50kD ectodomain (24-303aa) or soluble form.
Constitutive expression of Pref-1 or addition of its ectodomain inhibits adipogenesis.
There are at least four major isoforms (45-60kD) of Pref-1 generated by alternatively splicing.
Pref-1/DLK is expressed in tumors with neuroendocrine features, such as neuroblastoma, pheochromocytoma, and a subset of small cell lung carcinoma cell lines.
Its expression in normal tissues is restricted to the adrenal gland and placenta.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-5ug/ml using ECL
ELISA: 1:10,000-1:50,000.
Coat ELISA plates wtih 10-100ng of control peptide/well (P5899)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% BSA,,40% glycerol.
Specificity：Recognizes mouse Preadipocyte Factor 1. Because the eptiope is located near the cytoplasmic C-terminus, will not detect the ectodomain/soluble forms of Pref-1. Species Sequence Homology: human, rat: 93%; bovine: 87%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide consisting of 16aa sequence near the cytoplasmic, C-terminus of mouse Pref-1 (KLH).
