Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides.
Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need.
In order to accomplish these complex tasks of energy balance, adipocytes express many genes, including Pref-1, involved in lipid metabolism and glucose homeostasis
Preadipocyte factor-1 (Pref-1), an epidermal growth factor-like domain-containing transmembrane protein, is implicated in inhibiting preadipocytes differentiation.
Pref-1 (mouse 385-aa, rat 383-aa, human 383-aa, chromosome 14q32), is synthesized as a membrane protein in preadipocytes but it is not detectable in mature adipocytes.
Dexamethasone, a promoter of adipocyte differentiation, down-regulates Pref-1.
Pref-1 is proteolytically cleaved to generate a biologically active 50-kDa ectodomain (24-303 aa) or soluble form.
Constitutive expression of Pref-1 or addition of its ectodomain inhibits adipogenesis.
There are at least four major isoforms (45-60kD) of Pref-1 generated by alternate splicing.
Pref-1/DLK is expressed in tumors with neuroendocrine features, such as neuroblastoma, pheochromocytoma, and a subset of small cell lung carcinoma cell lines.
Its expression in normal tissues is restricted to the adrenal gland and placenta.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-50,000; 10-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: P5999: Preadipocyte Factor-1, Human, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 , 0.1% BSA.
Specificity：Recognizes mature human Preadipocyte Factor 1. Because the eptiope is located near the extracellular N-terminus, this antibody will detect both the membrane and soluble forms of human Preadipocyte Factor 1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa sequence near the extracellular, N-terminus of mature human Preadipocyte Factor 1. Species Sequence Homology: bovine and porcine: 84%; rat: 76%; mouse: 69%.
