84-1048-18　Period Clock Protein (dPER) (Drosophila) 50ul　P3342B

特徴

• Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms.
• In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm.
• Recently human and mouse genes encoding a basichelix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported.
• The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively.
• Homologues of mPer1 designated Per 2 (1257 aa) and Per3 (1113 aa) have also been cloned.
• Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes.
• It has been suggested that mPer regulates neuronal activity in the SCN.
• Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice.
• Clock has been mapped to chromosome 5.
• Mouse Clock encodes a transcription factor, a single polypeptide chain of 855 aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846 aa).
• Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney).
• Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery.
• Applications:Suitable for use in ELISA.
• Other potential application, though not tested is Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide:P3342A: Period Clock Protein, Rabbit, Control Peptide (dPER)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.