84-1048-09　Claudin 16 (Paracellin 1, CLDN16) 100ul　C5838-90C

特徴

• Magnesium as a cofactor is required in many cellular activities.
• Mg+2 reabsorption in the kidney is mediated primarily be a poorly understood paracellular pathway (passage of solutes between the cells) in the thick ascending limb of Henle (TAL).
• Tight junctions constitute the barrier to paracellular conductance.
• Familial hypomagnesaemia with hypercalciuria and nephrocalcinosis (FHHNC, MIM 248250) is a complex renal tubular disorder characterized by hypomagnesaemia, hypercalciuria, advanced nephrocalcinosis, hyposthenuria and progressive renal failure.
• The mode of inheritance is autosomal recessive.
• A primary defect in the reabsorption of magnesium in the TAL has been proposed to be essential in FHHNC pathophysiology.
• Recently, mutations in the gene paracellin-1 (Claudin 16) have been identified as the underlying genetic defect in FHHNC.
• Null mutation of Claudin-16 has been shown to produce chronic interstitial nephritis in cattle.
• PCLN-1 gene codes for a protein of 305 aa (chromosome 3q27), with four TM domains and intracellular NH2- and COOH-termini.
• Claudin-16 is only expressed in tight junction of TAL implicated in Mg+2-reabsorption.
• Applications:Suitable for use in ELISA, Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1-:1:3K) using ECL technique
• ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
• Control peptide can be used to coat ELISA plates at 1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.