Human p73a is the first known structural and functional homolog of one of the most studied tumor protein, p53 (1).
Human p73 gene encodes two distinct polypeptides: The p73 long form (human 636 AA, termed p73a; mouse 590 aa) and the short form (499 AA; termed p73b).
The short form arises through alternative splicing of the p73.
With the exception of C-terminal 5 AA, p73b is identical to p73a.
Both p73a and p73b transcripts were detectedall human tissues.
The endogenous p73a protein has also been detected in cell extracts of HT-29, IMR-32, and SK-N-SH cells.
Other cell lines, SK-N-MC SK-N-AS displayed significantly reduced levels of p73a protein (1).
The C-terminal domain (364-393) of p53 and p73 arequite distinct.
p73 has been mapped to human chromosome 1p36, a region that is frequently deleted in s variety of human cancers including neuroblastoma, colon cancer, melanoma, and breast cancer.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000
ELISA:1:10,000-50,000
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Recognizes human p73b. Species sequence homology: monkey. No significant homology with p73a.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：An 8 amino acid peptide sequence from the C-terminus of human p73b (KLH) (gene accession # BAB87245.1).
