Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control peptide, because of its low MW (<3kD), is not suitable for Western.
It should be used for ELISA or antibody blocking to confirm antibody specificity.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Affinity purified over the control-peptide Sepharose
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：The 8 AA human immunogenic peptide sequence has no significant homology with p73a. It is conserved in monkey p73. Antibody crossreactivity in various species is not established.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：An 8 amino acid peptide sequence mapping at the C-terminus of human p73b (1). The peptide was coupled with KLH.
