Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting ~ 1:500-1:2000 using ECL.
We recommend using recombinant or purified p53 that is acetylated by p300 HAT domain in an in vitro assay only.
It may not be suitable for detecting endogenous acetylated p53
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Specificity：Antibody recognizes only p53 acetylated at K373 residue. It does not react with non-acetylated p53 or unrelated proteins. It recognizes recombinant mouse and rat p53 as well. No crossreactivity is seen with p63 or p73. Antibody crossreactivity in various species is not established. Control immunogenic acetylated peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A peptide surrounding the human p53, acetylated at Lys373, coupled to KLH
