84-1047-93　Oxytocin 100ul　O8250-20

特徴

• The hypothalamic Oxytocin (OT) is a nine-amino acid peptide,which exerts multiple biological actions as a hormone and asneurotransmitter.
• OT stimulates uterine smooth muscle andmammary myoepithelial cell contraction, prostaglandin production by uterine endometrial and amnion cells, milk ejaculation from the mammary gland, and induction of specific mating behavior and maternal behaviors.
• Just before the onset of labor, uterine myometrium becomes extremely sensitive to oxytocin, for which it is a primary target tissue, because of a dramatic increase in the number of oxytocin receptors.
• OT initiates its physiological activity by interacting with the G protein-coupled receptor (GPCR) known as oxytocin receptor (OTR).
• The encoded receptor is a 388-amino-acid polypeptide with 7 transmembrane domains typical of G protein-coupled receptors.
• Messenger RNAs for the receptor are of two sizes, 3.6 kilobases in breast, and 4.4 kilobases in ovary, uterine endometrium and myometrium.
• The mRNA level in the myometrium is very high at term.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-50,000.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:O8250-25: Oxytocin, Rat Control Peptide
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.