IntroductionMammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion".
Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues.
OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
A kidney-specific anion transporter termed OAT-K1 (rat 669 aa) has been cloned from rat kidney.
OAT-K1 mRNA is specifically expressed in brush-borer membrane of the proximal straight tubules.
Rat OAT-K1 (669 aa) shows 72% identity with rat liver oatp.
It mediates basolateral uptake of anionic methotrexate but not PAH, taurocholate, and prostaglandin E2.
OAT-K2 (498 aa), very similar to OAT-K1 (91% identity), has also been cloned from rat kidney.
It also has 62-65% homology with oatp1-3 family of proteins.
OAT-K2 is predominantly expressed in convoluted tubules, proximal straight tubules, and cortical collecting ducts.
OAT-K2 mediates uptake of hydrophobic anions such as taurocholate, methotrexate, folate, and prostaglandin E2 suggesting that OAT-K2 participate in epithelial transport of hydrophobic anionic compound in the kidney.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution: Western Blot:1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL technique
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：The 16 AA rat control peptide is unique to rat OAT-K1. No significant sequence homology is detected with OAT-K2 or other OATs. The control peptide is available to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 14 AA Peptide sequence near the cytoplasmic, C-terminus of rat OAT-K1
