Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/wellWestern Blot: 1:1000-1:5000 using ECL
Optimal dilutions to be determined by the researcher.
Control Peptide:O7505-15: Orexin 2 Receptor, Human (Hypocretin 2 Receptor) (Control Peptide)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human OX2R. Species Sequence Homology: canine and porcine; 94%, rat; 84%, mouse; 78%. No significiant homology with OX1R or other G-protein-coupled receptors.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 19aa near the N-Terminal, extracellular domain of human Orexin 2 Receptor, conjugated to KLH.
