Several peptides associated with feeding behavior have been reported recently.
Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus.
Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Prepro-orexin is 130 amino acid long peptide with a putative 33-AA secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis.
These neuropeptides bind and activate two closely related Orexin receptors--G-protein coupled receptors (GPCRs) OX1R and OX2R.
Rat and human OX2R are 460 aa, and 444 aa, respectively.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng O7505-15 control peptide per well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA.
Specificity：Recognizes human OX2R. No significant homology is seen with OX1Ror other G-protein-coupled receptors.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide corresponding to 19aa near the N-terminus extracellular domain of human OX2R.
