Several peptides associated with feeding behavior have been reported recently.
Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1-2).
Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Prepro-orexin is 130 amino acid long peptide with a putative 33-AA secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis (1-2).
These neuropeptides bind and activate two closely related Orexin receptors--G-protein coupled receptors (GPCRs) OX1R and OX2R.
Rat and human OX2R are 460 aa, and 444 aa, respectively (1-2)
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.(see published refs)ELISA: 1:10,000-1:100,000 using 50-100ng of O7505-05 control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 40% glycerol.
Specificity：Rat is 94% homologous with mouse OX2R and 84% with canine and human. No significant homology is seen with OX1R or other G-protein-coupled receptors.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：19 aa peptide near the C-terminus, cytoplasmic domain. Coupled to KLH.
