84-1047-84　OGG1 (8-Oxoguanine DNA Glycosylase) 100ul　O5700-15

特徴

• Mouse 8-Oxoguanine DNA Glycosylase 1 (mOGG1; 345aa) is involved in the excision of 7, 8-dihydro-8-oxoguanine produced as a result of DNA damage.
• This gene has been localized on human chromosome 3p25.
• Several forms of hOGG protein have been cloned differing in lengths (351-424aa) (refs 1).
• 8-Oxoguanine- DNA glyosylase (OGG1) is a DNA repair protein that is primarily responsible for the repair of 8-oxoguanine (8-oxo-G).
• It possesses both DNA glycosylase and AP (apurinic) lyase activity and is a functional analog of the bacteria Fpg protein.
• OGG1 releases 8- oxoguanine (8-oxo-G) from mutated DNA and nicks the DNA strand.
• Although its glycosidase activity is very efficient, its lyase activity is weak and is highest for substrates having a C as the opposite base.
• OGG1 is a b-lyase generating an -unsaturated sugar moiety at the 3’-terminus.
• Murine OGG1 is a 345 amino acid protein of 38.8kD.
• It displays 84% identity to the human OGG1.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-1:50,000 using 50-100ng control peptide per well
• Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.