Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called “renal secretion”.
Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential differencethat governs the influx of cations, whereas the H+-gradient dependent transport system may mediate the efflux of organic cations.
Several multispecific, potential-sensitive transporters(OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues.
OCT superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and Cterminus.
OCT3 (Organic cation transporter 3; Synonyms Extraneuronal monoamine transporter, EMT, Solute carrier family 22 member 3, Gene name Name: SLC22A3; Synonyms: EMTH) rat/mouse 551 aa; human 556 aa) share 30% homology with OCT1 and 51% with OCT2.
It is most abundant in rat placenta, and moderate in the intestine, heart, and brain.
OCT3 expression is very low in kidney and lung and is undetectable in the liver.
OCT3 recognized TEA and guanidine along with other cations including neurotoxin (MPP0, neurotransmitter dopamine, and steroids.
OCT3 has beenidentified as extraneuronal monoamine transporter (uptake2).
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
ELISA:1:10,000-50,000.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：Recognizes rat OCT3. Species sequence homology: mouse, 100%; human, 94%; rabbit, 88%.No significant sequence homology is detected with other OCTs or OCTNs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：18-aa peptide from the mid region of rat OCT3 (KLH) (UniProt # O88446, refs 1)
