84-1047-81　Oct-3 (Organic Cation Transporter 3) 100ug　O5402-06

特徴

• Mammalian kidney and liver are critical in maintaining physiological ionic environment.
• Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion".
• Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations.
• Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues.
• The OCT superfamily of proteins shares a high degree of sequence homology and display up to 12 transmembrane domains with cytoplasmic N and C-terminus.
• OCT3 (rat/mouse 551 aa; human 556 aa) shares 30% homology with OCT1 and 51% with OCT2.
• It is most abundant in rat placenta, and moderate in the intestine, heart, and brain.
• OCT3 expression is very low in kidney and lung and is undetectable in the liver.
• OCT3 recognizes TEA and guanidine along with other cations including neurotoxin, MPP0, neurotransmitter dopamine, and steroids.
• OCT3 has been identified as an extraneuronal monoamine transporter (uptake 2).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
• ELISA: 0.5-1ug/ml using 1ug/ml control peptide per well
• Optimal dilutions to be determined by the researcher.
• Control peptide: O5402-05
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.