Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion".
Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations.
Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues.
OCT superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus.
Rat OCT2 was initially cloned from kidney by homology screening.
OCT2 (rat 593aa, mouse 553aa, human 555aa) shares ~70% homology with OCT1.
In rat, it is expressed primarily in kidney, and traces were found in colon stomach, and brain.
Rat OCT2 has been localized to the basolateral membrane of S2 and S3 segments of proximal tubules.
In contrast, OCT2 was localized to the luminal membranes of distal tubule.
OCT2 also mediated uptake of a variety of organic cations.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide (Cat #O5401-05) can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes rat Organic Cation Transporter 2. No significant sequence homology is detected with other Octs or OctNs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresonding to 21aa within the mid-region of rat Organic Cation Transporter 2 (KLH). Species Sequence Homology: mouse; 76%; porcine: 66%; human; 57%.
