84-1047-71　［取扱停止］Organic Anion Transporter 2 (OAT2) 100ug　O3003-11

特徴

• Mammalian kidney and liver are critical in maintaining physiological ionic environment.
• Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion".
• Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
• Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues.
• OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
• OAT2, previously called NLT (novel liver-specific transporter) shows 42% homology with OAT2.
• OAT2 (rat 535 aa; human 548 aa) is predominantly expressed in liver, and to a lower level in kidney.
• OAT2 mediates sodium-independent, multispecific organic anion transport (PAH, salicylate and acetylsalicylate, prostaglandin E2, and dicarboxylate).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL
• ELISA: 0.5-1ug/ml
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide: O3003-12.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.