Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion".
Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues.
OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
Rat Kidney PAH transporter, termed OAT1 or ROAT1, encodes a protein of 551 aa.
Human OAT1 (hOAT1) is 563 aa and its alternatively spliced isoform hOAT2 is 550 aa (missing 13 aa from 523-533 aa).
OAT1 has wide substrate selectivity, covering endogenous substrates such as cyclic nucleotides, prostaglandin and uric acid, and a variety of drugs (e.g., antibiotics, non-steroidal anti-inflammatory drugs, diuretics, anti-neoplastic drug, and a uricosuric drug).
OAT1 has been localized on the basolateral membrane of the proximal tubule in the kidney.
Weak expression was also detected in brain.
OAT1 has ~95 identity with mouse NKT, an ortholog of rat OAT1.
Applications:Suitable for use in ELISA Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL technique
ELISA: 0.5-1ug/ml Immunohistochemistry: 1-20ug/mlImmunofluorescence: 1-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rat Organic Anion Transporter 1. No significant sequence homology is detected with other OATs or other proteins
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Rt
Immunogen：A synthetic peptide corrsponding to 16aa near the cytoplasmic, C-terminus of rat OAT1, conjugated to KLH. Species Sequence Homology: mous and, rabbit; 87%, human; 81%.
