Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion".
Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues.
OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N- and C-terminus).
Oatp3 (670 aa; 12 TM), cloned from rat retina, shares ~80-83% identity with oatp1-2 and ~77% to rat OAT-K1.
Oatp3 is highly expressed in the kidney, and moderately in the retina.
Oatp3 mediates uptake of taurocholate, thyroxine, and triiodothyronine.
Most of the T4 secreted from the thyroid is deiodinated in peripheral tissues.
The liver and kidney are the major sites for the production of T3 and T4.
Therefore, oatp3 may play a critical role in transporting thyroid hormones from the circulation to the deiodination sites in the kidney.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:O3002-05: OATP3, Rat, Control Peptide (Organic Anion Transporting Polypeptide 3)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS. pH 7.2, 0.1% BSA.
Specificity：Recognizes rat Oatp3. No significant sequence homology with other Oatp2, Oatp3 or human OATP or LST-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide consisting of 12aa sequence within the mid-region of rat Oatp3 conjugated with KLH. Species Sequence Homology: mouse Oatp3: 75%.
