84-1047-65　OATP2 (Organic Anion Transporting Polypeptide 2) 100ul　O3001-10

特徴

• Mammalian kidney and liver are critical in maintaining physiological ionic environment.
• Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called “renal secretion”.
• Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
• Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues.OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
• Oatp2 (also called OATP-B1; 661 aa; 12 TM), cloned from rat brain, shares significant homology with rat oatp1 and OAT-K1 (77%) and human OATP (73%).
• It is highly expressed in brain, liver, and kidney but not in heart, spleen, lung, muscle, and testes.
• Oatp2 mediates uptake of taurocholate, estrogen conjugates, and cardiac glycosides..
• Unlike other oatp’s, oatp2 also transported digioxin.
• Applications:Suitable for use in ELISA.
• Although not tested, Western Blot may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-50,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.