Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs and other organic anions from the blood by a process called “renal secretion.” Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway and placenta.
Several multispecific OATs (OAT1-3, OAT-K1, OATK2) and OATPs (oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues.
OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
OAT2, previously called NLT (novel liver-specific transporter) shows 42% homology with OAT2.
OAT2 (rat 535 aa; human 548 aa) is predominantly expressed in liver, and to a lower level in kidney.
OAT2 mediates sodium-independent, multispecific organic anion transport (PAH, salicylate and acetylsalicylate, prostaglandin E2, and dicarboxylate).
Applications:Suitable for use in ELISA and Immunohistochemistry.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
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Recommended Dilutions:ELISA: 0.5-1ug/mlImmunohistochemistry (Paraformaldehyde fixed): 2-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% BSAReconstitute with 100ul sterile PBS.
Specificity：Recognizes rat OATP2 (Organic Anion Transporting Polypeptide 2). No significant sequence homology is detected with other OATP1 and OATP3 or human OATP or LST-1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthertic peptide corresponding to 12aa near the C-terminus of rat OATP2, conjugated to KLH. Species Sequence Homology: mouse; 50%
