84-1047-63　OATP1 (Organic Anion Transporting Polypeptide 1) 100ul　O3000-06

特徴

• Mammalian kidney and liver are critical in maintaining physiological ionic environment.
• Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion".
• Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta.
• Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues.
• OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).
• Rat Oatp, now called oatp1, encodes a protein of 670 aa.
• Oatp1 was localized at the apical portions of the S3 segment of the proximal tubule, and at the apical surface of the choroid plexus of rat brain.
• It is also found in liver, lung, skeletal muscle, and colon.
• It mediates Na+-independent uptake of wide range of amphipathic substrates including sulfobromopthalein (BSP), bile acids, estrogen conjugates, and neutral steroids, etc..
• Human liver OATP (670aa) is only 67% homologous with rat oatp1.
• It differs in functional properties from rat oatp1 and highly expressed in brain.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-1:50,000 Control peptide (Cat #O3000-05) can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.