84-1047-59　NP-6 (alpha Defensin-6) 100ul　N5378-08D

特徴

• Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
• Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-sheet structure.
• Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.
• In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
• The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23.
• Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides.
• beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides.
• In humans, six alpha-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified.
• Alpha-defensins are encoded by genes designated DEFA1-6, whereas human beta-defensins are encoded by the DEFB1 and DEFb2 genes.
• HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract.
• HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein.
• The HNPs are 29-30 amino acids long and are identical in sequence except at the N-terminal amino acid.
• DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively.
• DEFA2 is identical to both except that it has 29 instead of 30 amino acids.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
• ELISA: 1:10,000-1:50,000.
• Coat with 1ug/ml with control peptide
• Optimal dilutions to be determined by the researcher.
• Control peptide: N5378-08
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.