Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure.
Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.
In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides.
B-defensins are slightly larger and differ in the position and arrangement of 3 disulfides.
In humans, six a-defensin (cryptidins), HD1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.
Alpha-defensins are encoded by genes designated DEFA1-6, whereas human b-defensins are encoded by the DEFB1 and DEFb2 genes.
HD1-4 are expressed in neutrophils, whereas HD5 and HD6 are expressed in epithelial cells of the intestinal and reproductive tract.
HD1-3/HNP1-3 make up about 30% of the neutrophil's total granule protein.
The HNPs are 29-30aa long and are identical in sequence except at the N-terminal aa.
DEFA1 and DEFA3 differ from each other only by their terminal ala and asp, respectively.
DEFA2 is identical to both except that it has 29 instead of 30aa.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml; Control peptide (N5378) can be used to coat ELISA plates at 1ug/ml
Western Blot: 1-10ug/ml using ECL.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA.Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human NP-4 (alpha Defensin-4). No significant homology exists with other alpha-defensins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa near the C-terminus of mature human alpha Defensin-4, conjugated to KLH.
