84-1047-55　NP-4 (alpha Defensin-4) 100ul　N5378-05

特徴

• Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
• Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure.
• Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.
• In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
• The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23.
• Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides.
• b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides.
• In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000ELISA: 1:10,000-50,000.
• N5378 Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.