84-1047-52　Novel Organic Cation Transporter 1 (OctN1) 100ul　N5376-52

特徴

• Mammalian kidney and liver are critical in maintaining physiological ionic environment.
• Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion".
• Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations.
• Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues.
• Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus.
• A novel transporter termed OctN1, has been cloned and characterized human fetal liver, which carries a nucleotide birding site motif.
• OctN1 (human 551aa, mouse 55aa) shares ~32% homology with Oct1-3.
• It is strongly expressed in adult kidney, trachea, bone marrow, and fetal liver, and several tumor cells, but not in adult human liver.
• OctN1 mediated uptake of TEA in pH dependent manner.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000 using ECL
• ELISA: 1:10,000-1:50,000.
• Control peptide can be used to coat ELISA plates at 1ug/mlOptimal dilutions to be determined by the researcher.
• Control Peptide:N5376-50: Novel Organic Cation Transporter 1, Mouse, Control Peptide (OctN1)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.