Mammalian kidney and liver are critical in maintaining physiological ionic environment.
Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion".
Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations.
Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues.
Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N- and C-terminus
A novel transporter termed OctN1, has been cloned and characterized from human fetal liver, which carries a nucleotide birding site motif.
OctN1 (human 551aa, mouse 553aa) shares ~32% homology with Oct1-3.
It is strongly expressed in adult kidney, trachea, bone marrow, and fetal liver, and several tumor cells, but not in adult human liver.
OctN1 mediated uptake of TEA in pH dependent manner.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
Coat ELISA plates with control peptide at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:N5376-50: Novel Organic Cation Transporter 1, Mouse, Control Peptide (OctN1)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes mouse Novel Organic Cation Transporter 1. No significant sequence homology is detected with other Octs or OctNs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 20aa within the C-terminus of mouse OctN1, conjugated to KLH (Accession# NP_066261.1). Control peptide is 88% conserved in rat and 61% in human OCTN1.
