Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system.
It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium.
Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors.
Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.
NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS , I-III, ~130-160kD).
One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA).
It is found in endothelium, adrenal glands, brain and platelets.
The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA.
It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.
Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).
Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible.
Sequence homology among different cloned isoforms is ~50%.
Applications:Suitable for use in ELISA, Western Blot, Antibody competition Assays.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000+ELISA: Control peptide (N5350-05D) should be coated at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.09% sodium azide before the addition of 40% glycerol.
Specificity：Recognizes bNOS/NOS-1. Species crossreactivity: Human (1433aa), rat (1429aa), mouse (1429aa) and rabbit (1). No significant sequence homology is seen with NOS-2/NOS-3 or other proteins.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acid sequence 724-739 of human brain NOS was synthesized and coupled to KLH (1).
