84-1047-45　Norepinephrine Transporter (NET) 100ul　N3950-05

特徴

• Noradrenaline transporter or epinephrine transporter (NET or NTT1 or NAT1 or SLC6A2) gene (chromosome 16) encodes a protein of 617aa (mouse 617aa, ~94% identity), with 12-13 transmembrane domains, and cytoplasmic N and C-termini.
• Expression of the cDNA clone in transfected HeLa cells indicated that noradrenaline transport activity is sodium-dependent and sensitive to selective noradrenaline transport inhibitors.
• NET mRNA has been localized to the brain stem and adrenal.
• The predicted protein sequence demonstrated significant amino acid identity with the Na(+)/gamma-aminobutyric acid transporter, thus identifying a new gene family for neurotransmitter transport proteins.
• Alternative splicing of NET mRNA produces two transporter isoforms, rNETa and rNETb, which differ at their COOH termini.
• The rNETa isoform reveals a COOH terminus homologous to human NET and transports norepinephrine.
• In contrast, rNETb revealed no detectable transport function, but reduced functional expression of rNETa when both isoforms were expressed in the same cell.
• Defects in NET are a cause of orthostatic intolerance (OI), a syndrome characterized by lightheadedness, fatigue, altered mentation and syncore.
• It is associated with postural tachycardia.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000
• ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide: N3950-06
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.