Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL
ELISA: ~1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control peptide, because of the small size (2-3kD), is not recommended for Western.
It should be used in ELISA or antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of affinity purified IgG or 1 ul of antiserum) to demonstrate antibody specificity
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 20-aa rat sequence has no significant sequence homology with human NMUR2. No significant sequence homology exist with NMUR1 or Ghrelin receptors or other proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 16 AA peptide within the extracellular, N-terminal domain or human, and a 20-aa peptide mapping at the cytoplasmic, C-terminal domain of rat NMUR2 (1
