The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction.
Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction).
Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission.
Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), have been identified as cognate receptors of NmU.
NMU receptors display a typical 7 TM domains with extracellular N-terminus and intracellular C-terminus.
NMUR1 has moderate sequence homology with neurotensin (NT), and growth hormone secretagogue (GHS, Ghrelin) receptors.
NMUR1 is specifically activated by the three peptides (NmU-25, NmU23, and NmU-8) with more or less the same potency.
Calcium mobilization assay suggests that NMUR1 is coupled to G-protein of the Gq/11 subfamily.
It is highly expressed in rat small intestine and lung.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml ECL
ELISA: 1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:N2911-15: NMU Receptor 1, Human Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes human NMUR1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to the 20aa at the cytoplasmic, C-terminal domain of human NMUR1 (KLH). Species Sequence Homology: mouse and rat, 65%.
