The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction.
Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction).
Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission.
Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), have been identified as cognate receptors of NmU.
NMU receptors display a typical 7 TM domains with extracellular N-terminus and intracellular C-terminus
The human NMUR1 (rat 402 aa; mouse, 405 aa; human 403 aa, chromosome 2; 70-80% interspecies homology) orphan GPCR66 was originally identified by similarity to mouse orthologue FM-3.
NMUR1 has moderate sequence homology with neurotensin (NT), and growth hormone secretagogue (GHS, Ghrelin) receptors.
NMUR1 is specifically activated by the three peptides (NmU-25, NmU23, and NmU-8) with more or less the same potency.
Calcium mobilization assay suggests that NMUR1 is coupled to G-protein of the Gq/11 subfamily.
It is highly expressed in rat small intestine and lung.
In human, highest expression was found in intestine adipose tissue, with moderate levels of expression in the intestine, lymphocytes, stomach, pancreas, bone marrow, and spleen, and low levels in most other tissues including brain.
Applications:Suitable for use in ELISA, Western Blot and Immunoistochemistry.
ELISA: 1:100,000 using 50-100ng control peptide/well (Cat #N2911-05)
Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissueOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Recognizes rat NMU Receptor 1 (NMUR1).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 20aa near the N-terminus, extracellullar domain of rat NMU Receptor 1 (NMUR1), conjugated to KLH (NP_075588.1). Species Sequence Homology: mouse and human, 65%
