84-1047-34　［取扱停止］Nitrotyrosine (nTyr) 100ug　N2700-02

特徴

• Nitric oxide (NO) and reacti04.10.07ve oxygen species (ROS) are important mediators that produce harmful or protective functions.
• NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-).
• Peroxynitrite induces nitration of tyrosine residues leading to changes in protein structure and function, and alterations in signaling pathways.
• Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-5ug/mlELISA: 1:10,000-1:50,000 using 50-100ng control nitrated proteins/well.
• Optimal dilutions to be determined by the researcher.
• Western Blot Nitrated MW Standards: N2700-10
• Positive Control for Western Blot:Proteins extracted from human A431 cells or other cells pretreated with peroxynitrite for 5 min
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.