Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that influences distinct central and peripheral physiological functions in mammals.
Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion.
NT is widely distributed throughout the CNS.
It has been localized to catecholamine-containing neurons.
NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2).
Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins.
All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13).
Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1
Most recently, a novel, non-GPCR, NTR3/sortilin/gp95/NT3 (mouse 825 aa, rat 748 aa; human 833 aa, chromosome 1p21.3; ~92% identity between human and mouse) also binds NT.
Sortilin was initially isolated as by its affinity for receptor-associated protein (RAP).
NTR3, the first non-GPCR neuropeptides receptor, sequence includes a putative secretory signal peptide, a furin cleavage site, and a single transmembrane domain near the C-terminus.
The mature protein has 44 N-terminal residues removed during processing.
It is homologous to the yeast protein Vps10p and the human sortilin-related receptor.
The C terminus of the protein contains a lysosomal sorting motif shared by the mannose-6-phosphate receptors.
NTR3 is expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta, and testis.
Applications:Suitable for use in ELISA.
Other potential application, though not tested is Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2177-50: Neurotensin Receptor 3, Control Peptide, Human (NTR3, Sortilin)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human Neurotensin Receptor 3. No significant sequence homology exists with NTR1/NTR2 or other GPCR.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide sequence corresponding to 18aa close to the extracellular, N-terminus region of human NTR3 conjugated to KLH. Species Sequence Homology: mouse and rat NTR3: 94%
