Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that influences distinct central and peripheral physiological functions in mammals.
Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion.
NT is widely distributed throughout the CNS.
It has been localized to catecholamine-containing neurons.
NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2).
Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins.
All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13).
Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1.
NTR1/NTRH/NTSR1/NT1 (mouse/rat 424 aa; human 418 aa, chromosome 20q1; ~84% interspecies sequence homology) is the levocabastine-insensitive, high affinity receptor for NT.
It has the typical structure of GPCR: 7 TM domains with extracellular N-terminus and cytoplasmic C-terminus.
It is shown to mediate a number of peripheral and central NT responses, including the neuroleptic-like effects of the peptide.
It is highly expressed in the brain, small intestine, and other peripheral tissues.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
NTR1 is ~52-55kD in rat brain and it may be differentially glycosylated
ELISA: 1:100,000 using 50-100ng N2177-20 control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium sodium azide, 40% glycerol.
Immunogen：An 18aa peptide sequence within the mid-region, extracellular location of mouse NTR1. (KLH). Species sequence homology: 84% rat and human. No significant sequence homology exist with NTR2 or NTR3 or other GPCR.
