Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter (pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg- pro-Tyr-Ile-Leu-OH) that was originally isolated from bovine hypothalamus.
NT influences distinct central and peripheral physiological functions in mammals.
Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion.
NT causes contraction of smooth muscle.
NT is widely distributed throughout the CNS.
It has been localized to catecholamine-containing neurons.
NT gene (human chromosome 12q21) produces both NT and neuromedin N (NN).
In the rat, the NT precursor consists of a 169-aa polypeptide.
The signal peptide (1-22 aa) is removed to produce a peptide that is further processed to produce NN (142-147 aa) and NT (150-162 aa).
NT sequence is completely conserved in mouse, rat, human, canine, bovine etc.
NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2).
Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins.
All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13).
Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:100,000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug/4mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, 0.1% BSA
Specificity：Recognizes human Neurotensin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide, 13aa corresponding to the N terminal of human Neurotensin. Species sequence homology: mouse, rat, bovine and canine 100%; guinea pig 92%. No significant sequence homology exists with other neuromedins.
