84-1047-22　Neurotensin, NT (Human Proneurotensin, Large Neuromedin N, Neuromedin N Preproprotein, NMN-125, NN, NT, NT/N, NTRH, NTS, NTS1, Proneuromedin N mRNA, Tail Peptide) 100ul　N2177-10

特徴

• Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter (pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg- pro-Tyr-Ile-Leu-OH) that was originally isolated from bovine hypothalamus.
• NT influences distinct central and peripheral physiological functions in mammals.
• Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion.
• NT causes contraction of smooth muscle.
• NT is widely distributed throughout the CNS.
• It has been localized to catecholamine-containing neurons.
• NT gene (human chromosome 12q21) produces both NT and neuromedin N (NN).
• In the rat, the NT precursor consists of a 169-aa polypeptide.
• The signal peptide (1-22 aa) is removed to produce a peptide that is further processed to produce NN (142-147 aa) and NT (150-162 aa).
• NT sequence is completely conserved in mouse, rat, human, canine, bovine etc.
• NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2).
• Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins.
• All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13).
• Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL
• ELISA: 1:100,000 using 50-100ng control peptide/well
• Optimal dilutions to be determined by the researcher.
• Control Peptide: N2177-09
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.