The peripheral nerve regeneration entails sequential changes in the expression of thousands of genes.
These changes are necessary to protect the damaged neurons from death, activate the surrounding glial cells, and accelerate the neurite elongation.
The nerve regeneration requires a wide range of active proteases including few members of the M-13 metalloprotease family.
The M-13 family comprises several zinc-dependent metalloproteases like (DINE), PHEX, KELL, ECE, XCE, neprilysin (NEP) and neprilysin-like proteases (NEPLs).
The NEPLs (NEPL-alpha, NEPL-alpha, NEPL-gamma) arise from the alternative splicing of a single NEPL gene and are zinc dependent metalloproteases with ~54% homology to NEP.
DINE (damage-induced neuronal endopeptidase) or Endothelin converting enzyme-like 1 (ECEL1) or X-converting enzyme (Xce) is a 95kD (mouse/rat/human 775aa, chromosome 2q36-q37), type II integral membrane metalloprotease containing a conserved zinc-binding motif and an ENXADX consensus sequence, consistent with gluzinitin.
The aa sequence of DINE is 36 and 32% identical to ECE-1 and NEP, respectively.
But, DINE is devoid of enzyme activity like ECE.
Unlike NEP, DINE has no proteolytic activity to A beta.
However, the enzyme can hydrolyze synthetic NEP substrates and thiorphan, EDTA and phosphoramidon inhibit its activity.
DINE also inhibits C2-ceramide induced apoptosis in COS-7 cells.
Although, the endogenous substrate for DINE is yet to be identified, its proteolytic activity activates, at least in part, free radical scavenging in damaged neurons.
The DINE expression is restricted to brain tissue with predominant expression in hypothalamus, large cholinergic cells in striatum and low expression in virtually all regions of brain except in cerebral cortex, hippocampus and cerebellum.
DINE, expression is markedly increased in response to optic, spinal sensory, cortical and thalamic nerve injury.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2174-50: Neuronal Endopeptidase, Rat
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes rat DINE.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 17aa peptide sequence, mapping near the cytoplasmic N-terminus of rat DINE was synthesized, coupled to KLH. Species sequence homology: Mouse (94%) and human (88%). No significant sequence homology is seen with NEP, NEPLs or ECEs or other proteins.
