Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrow after injury.
The CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses.
Most recently an inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized.
It may help block the regeneration of the CNS.
Nogo is the 4th member of reticulon (Rtn) family.
There are three alternative isoforms of Nogo, designated Nogo-A (full length), an intermediate form Nogo-B (373aa; ~37 K, lacks 186-1004aa within the extracellular domain), and a shorter form Nogo-C (199aa; ~25 K, similar to rat vp20 and foocen-s; lacks 186-1004aa but which has a smaller, alternative N-terminal domain).
A 66aa hydrophilic region of Nogo (Nogo-66), located between the two TM domains, has the most inhibitory properties of Nogo.
In contrast to Nogo, Rtn 1, -2 and 3 do not inhibit axonal regeneration.
This 66aa region also has the least similarity to Rtn proteins.
The corresponding Rtn-P4 peptide sequence has no activity.
A brain specific leucine-rich-repeat protein with high affinity for soluble Nogo-66, termed Nogo receptor (Ngr) has been cloned and characterized.
Cleavage of Ngr from the axonal surface renders neurons insensitive to Nogo-66.
Ngr expression is sufficient to impart Nogo-66 axonal inhibition to unresponsive neurons.
Ngr protein (mouse, rat, monkey, and human 473aa; chromosome 22q11) contains a signal sequence followed by eight LRR domains, an LRR cysteine-rich CT-flanking domain, and unique GPI anchorage site.
Human and mouse Ngr proteins are ~89% identical.
Ngr is expressed in brain and lower levels are also detected in hear and kidney but not in other peripheral tissues.
Ngr has been localized to axons.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2116-95C: Nogo 66 Receptor, Human (Ngr)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 005% sodium azide, 40% glycerol.
Specificity：Recognizes Nogo 66 Receptor. No significant sequence homology is detected with Rtn family members. Species sequence homology: Human, mouse, rat and monkey Ngr: 100%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 17aa within the C-terminus of human Ngr (KLH coupled).
