IntroductionMany tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrowafter injury.
However, the CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses.
Most recently an inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized.
It may help block the regeneration of the CNS.
Nogo is the 4th member of reticulon (Rtn) family.
There are three alternative isoforms of Nogo, designated Nogo-A (full length human protein 1192 aa; calculated mol wt 135kD; rat 1163 aa), an intermediate form Nogo-B (373 aa; ~37 K, lacks 186-1004 aa within the extracellular domain), and a shorter form Nogo-C (199 aa; ~25 K, similar to rat vp20 and foocen-s; lacks 186-1004 aa but which has a smaller, alternative N-terminal domain).
Nogo-A has a putative extracellular domain of 1024aa, 2-3 TM domains, and a short C-terminus of 43aa.
Nogo-A is localized to the CNS-myelin, and is highly expressed in oligodendorcytes but not by Schwann cells.
Nogo-B and Nogo-C have been found in several non-neuronal tissues (skeletal muscle, kidney, skin, lung, and spleen), and it may be the 35-kD protein recognized by IN-1 antibody.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting(1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
Nogo-C has a MW of ~25kD (1)
ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The human peptide sequence is conserved in all 3 isoforms (Nogo A-C). It is 92% identical in rat Nogo or rat foocen-m1/2 protein. Within the 25-aa region identified for the inhibition of neurite outgrowth (1). Antibody crossreactivity of NogoA12 in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 13-aa peptide between the putative 2nd-3rd transmembrane domain of human Nogo-A (1) conjugated to KLH
