Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10ug/ml using ECLOptimal dilutions to be determined by the researcher.
Control Peptide: N2116-61
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:Recognizes Neurite Outgrowth Inhibitor Protein C (NogoC). Species Sequence Homology: human Nogo-C/foocen-S/RTN 4: 100%; mouse Nogo-C/foocen-S/RTN 4: 81%. Most of this region is unique to Nogo-C and not conserved in Nogo-A/B isoforms.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Rt
Immunogen:An 11aa peptide within the N-terminal, extracellular domain of human Nogo-C.(KLH coupled).