Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrowafter injury.
The CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses.
An inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized.
It may help block the regeneration of the CNS.
Nogo is the 4th member of reticulon (Rtn) family.
There are three alternative isoforms of Nogo.
Nogo-A is full length human protein consisting of 1192aa, Mr ~135kD (rat 1163aa).
Nogo-B is an intermediate form consistiong of 373aa, ~37kD.
Nogo-B lacks aa186-1004 within the extracellular domain.
Nogo-C is shorter consisting of 199aa, ~25kD.
It lacks aa186-1004 and has a smaller, alternative N-terminal domain.
It is similar to rat vp20 and foocen-s.
Nogo-A has a putative extracellular domain of 1024aa, 2-3 TM domains, and a short C-terminus of 43aa.
Nogo-A is localized to the CNS-myelin.
It is highly expressed in oligodendorcytes, but not by Schwann cells.
Nogo-B and Nogo-C have been found in several non-neuronal tissues (skeletal muscle, kidney, skin, lung, and spleen).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
Nogo-B has a MW of ~37kD (1)
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2116-41: Neurite Outgrowth Inhibitor Protein B, Rat (Nogo-B)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes Neurite Outgrowth Inhibitor Protein B. Peptide sequence was slected at the splice site of Nogo-B in a manner that the complete 12aa epitope is only present in Nogo-B. This region in absent in Nogo-C. Species Sequence Homology: Mouse: 100%; Human (Nogo-B/Foocen-m/foocen-m2): 83%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide 12aa within the N-terminus and at the splice site of Nogo-A from the putative extracellular domain of rat Nogo-B (KLH coupled).
