Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:N2116-21: Neurite Outgrowth Inhibitor Protein A (NogoA), Control Peptide
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:Recognizes rat Neurite Outgrowth Inhibitor Protein A (NogoA). Epitope is not present in alternatively spliced shorter forms of Nogo-B and Nogo-C. No significant sequence homology is detected with Rtn family members.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Rt
Immunogen:KLH-conjugated synthetic peptide mapping to a 18aa peptide near the middle region, cytoplasmic domain of rat Neurite Outgrowth Inhibitor Protein A. Species Sequence Homology: mouse: 94%; human: 83%